CHEK1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
Gene Name
Checkpoint kinase 1
Gene Aliases
C85740; checkpoint kinase 1 homolog; Checkpoint kinase-1; Chk1; CHK1 checkpoint homolog; rad27; rad27 homolog; serine/threonine-protein kinase Chk1.
Gene ID
12649
Accession Number
NP_031717.2
Reactivity
Mouse, Mus musculus
Target
Serine/threonine-protein kinase Chk1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CHEK1 ELISA Kit (Mouse) (OKEH07727) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHEK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHEK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHEK1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103% (n = 20)
Sensitivity
0.056ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Serine/threonine-protein kinase Chk1
NCBI Gene Symbol
Chek1
Protein Name
O35280
Gene Name URL
Chek1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_007691.5
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
