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IFI30 ELISA Kit (Bovine)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

IFI30 lysosomal thiol reductase

Gene Aliases

Gamma-inducible protein 30; gamma-interferon-inducible lysosomal thiol reductase; IFI30 protein; interferon, gamma-inducible protein 30.

Gene ID

615930

Accession Number

NP_001094721.1

Reactivity

Bos taurus, Bovine

Target

Gamma-interferon-inducible lysosomal thiol reductase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology IFI30 ELISA Kit (OKEH07716) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-IFI30 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated IFI30 and incubated. The IFI30 found in the sample or standards competes with the biotinylated IFI30 for limited binding sites on the immobilized anti-IFI30 antibody. Excess unbound biotinylated IFI30 and sample or standard IFI30 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated IFI30 captured in the well and inversely proportional to the amount of IFI30 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20)

Sensitivity

0.099ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Bovine Gamma-interferon-inducible lysosomal thiol reductase

NCBI Gene Symbol

IFI30

Protein Name

A6QPN6

Gene Name URL

IFI30

Nucleotide Accession Number

NM_001101251.2

Frequently Asked Questions

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