What is the catalog number for NAAA ELISA Kit (Rat)?

The catalog number for NAAA ELISA Kit (Rat) is OKEH07709-96W.

What sizes are available for NAAA ELISA Kit (Rat)?

NAAA ELISA Kit (Rat) is available in the following size(s): 96 Tests.

Is NAAA ELISA Kit (Rat) in stock?

NAAA ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

NAAA ELISA Kit (Rat)

Selected Variant

OKEH07709-96W

Size: 96 Tests

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

N-acylethanolamine acid amidase

Gene Aliases

Acylsphingosine deacylase NAAA; Asahl; ASAH-like protein; N-acylethanolamine-hydrolyzing acid amidase; N-acylsphingosine amidohydrolase-like.

Gene ID

497009

Accession Number

NP_001010967.1

Reactivity

Rat, Rattus norvegicus

Target

N-acylethanolamine-hydrolyzing acid amidase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NAAA ELISA Kit (Rat) (OKEH07709) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NAAA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NAAA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NAAA captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)

Sensitivity

39.23pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat N-acylethanolamine-hydrolyzing acid amidase

NCBI Gene Symbol

Naaa

Protein Name

Q5KTC7

Gene Name URL

Naaa

Nucleotide Accession Number

NM_001010967.2

Price

Frequently Asked Questions

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