What is the catalog number for UBE2I ELISA Kit (Chicken)?

The catalog number for UBE2I ELISA Kit (Chicken) is OKEH07701-96W.

What sizes are available for UBE2I ELISA Kit (Chicken)?

UBE2I ELISA Kit (Chicken) is available in the following size(s): 96 Well.

Is UBE2I ELISA Kit (Chicken) in stock?

UBE2I ELISA Kit (Chicken) is currently made to order / not held in immediate stock. Contact us for lead time.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

UBE2I ELISA Kit (Chicken)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Ubiquitin conjugating enzyme E2 I

Gene Aliases

RING-type E3 SUMO transferase UBC9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; UBC9; UBCE9; ubiquitin carrier protein 9; ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) ; ubiquitin-protein ligase I.

Gene ID

374123

Accession Number

NP_989596.1

Reactivity

Chicken, Gallus gallus

Target

SUMO-conjugating enzyme UBC9

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology UBE2I ELISA Kit (Chicken) (OKEH07701) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UBE2I has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UBE2I is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UBE2I captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% (n = 20)

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Chicken SUMO-conjugating enzyme UBC9

NCBI Gene Symbol

UBE2I

Protein Name

P63283

Gene Name URL

UBE2I

Nucleotide Accession Number

NM_204265.1

Frequently Asked Questions

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