Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

FZD1 ELISA Kit (Chicken)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Frizzled class receptor 1

Gene Aliases

7-transmembrane protein frizzled-1; cFz-1; frizzled family receptor 1; frizzled homolog 1; frizzled-1; Fz-1.

Gene ID

374062

Accession Number

NP_001025508.1

Reactivity

Chicken, Gallus gallus

Target

Frizzled-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FZD1 ELISA Kit (Chicken) (OKEH07698) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FZD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FZD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FZD1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 119% (n = 20)

Sensitivity

0.172ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Chicken Frizzled-1

NCBI Gene Symbol

FZD1

Protein Name

O57328

Gene Name URL

FZD1

Nucleotide Accession Number

NM_001030337.1

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

Mucin 5AC (MUC5AC) (45M1), CF568 conjugate, 0.1mg/mL
BNC680031-500 1x 500 µL

Mucin 5AC (MUC5AC) (45M1), CF568 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD47 (B6H12.2), CF647 conjugate, 0.1mg/mL
BNC470437-500 1x 500 µL

CD47 (B6H12.2), CF647 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD44 Standard (156-3C11), CF568 conjugate, 0.1mg/mL
BNC680460-100 1x 100 µL

CD44 Standard (156-3C11), CF568 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD20 / MS4A1 (B-Cell Marker) (rIGEL/773), CF405S conjugate, 0.1mg/mL
BNC041829-100 1x 100 µL

CD20 / MS4A1 (B-Cell Marker) (rIGEL/773), CF405S conjugate, 0.1mg/mL

Sign In for Pricing
View Details
Histone H1 (1415-1), CF740 conjugate, 0.1mg/mL
BNC740580-100 1x 100 µL

Histone H1 (1415-1), CF740 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
C-Myc (MYC275), CF740 conjugate, 0.1mg/mL
BNC740275-500 1x 500 µL

C-Myc (MYC275), CF740 conjugate, 0.1mg/mL

Sign In for Pricing
View Details