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DEFB104A ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Defensin beta 104A, defensin beta 104B

Gene Aliases

BD-4; beta-defensin 104; beta-defensin 4; DEFB104; DEFB4; DEFB-4; defensin, beta 104; defensin, beta 4; hBD-4.

Gene ID

140596, 503618

Accession Number

NP_001035792.1

Reactivity

Homo sapiens, Human

Target

Beta-defensin 104

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DEFB104A, DEFB104B ELISA Kit (Human) (OKEH07649) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEFB104A, DEFB104B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DEFB104A, DEFB104B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEFB104A, DEFB104B captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)

Sensitivity

0.12ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Beta-defensin 104

NCBI Gene Symbol

DEFB104A, DEFB104B

Protein Name

Q8WTQ1

Gene Name URL

DEFB104A, DEFB104B

Nucleotide Accession Number

NM_001040702.1

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