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DFFB ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

DNA fragmentation factor subunit beta

Gene Aliases

CAD; caspase-activated deoxyribonuclease; caspase-activated DNase; caspase-activated nuclease; CPAN; DFF2; DFF40; DFF-40; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) .

Gene ID

1677

Accession Number

NP_001307061.1

Reactivity

Homo sapiens, Human

Target

DNA fragmentation factor subunit beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DFFB ELISA Kit (Human) (OKEH07623) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DFFB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DFFB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DFFB captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

125-8000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110% (n = 20)

Sensitivity

62.5pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human DNA fragmentation factor subunit beta

NCBI Gene Symbol

DFFB

Protein Name

O76075

Gene Name URL

DFFB

Nucleotide Accession Number

NM_001320132.1

Frequently Asked Questions

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