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Hspa12a ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Heat shock protein 12A

Gene Aliases

1700063D12Rik; AI118035; AI840429; AW048913; AW556406; D5Mgi4; D5Mgi40; Gm19925; heat shock 70 kDa protein 12A; heat shock 70kDa protein 12A; Hsp; mKIAA0417.

Gene ID

73442

Reactivity

Mouse, Mus musculus

Target

Heat shock 70 kDa protein 12A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Hspa12a ELISA Kit (Mouse) (OKEH07582) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hspa12a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hspa12a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hspa12a captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106% (n = 20)

Sensitivity

0.082ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Heat shock 70 kDa protein 12A

NCBI Gene Symbol

Hspa12a

Protein Name

Q8K0U4

Gene Name URL

Hspa12a

Frequently Asked Questions

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