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MYL12A ELISA Kit (Bovine)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Myosin, light chain 12A, regulatory, non-sarcomeric

Gene Aliases

Fast skeletal myosin light chain 2; MRCL3; MRLC2; MYL9; Myosin regulatory light chain 2, smooth muscle isoform; myosin regulatory light chain 9; myosin regulatory light chain MRCL3; myosin regulatory light polypeptide 9.

Gene ID

531505

Accession Number

NP_001015640.1

Reactivity

Bos taurus, Bovine

Target

Myosin regulatory light polypeptide 9

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MYL12A ELISA Kit (Bovine) (OKEH07563) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MYL12A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MYL12A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MYL12A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Bovine Myosin regulatory light polypeptide 9

NCBI Gene Symbol

MYL12A

Protein Name

Q5E9E2

Gene Name URL

MYL12A

Nucleotide Accession Number

NM_001015640.2

Frequently Asked Questions

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