MYL12A ELISA Kit (Bovine)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Myosin, light chain 12A, regulatory, non-sarcomeric
Gene Aliases
Fast skeletal myosin light chain 2; MRCL3; MRLC2; MYL9; Myosin regulatory light chain 2, smooth muscle isoform; myosin regulatory light chain 9; myosin regulatory light chain MRCL3; myosin regulatory light polypeptide 9.
Gene ID
531505
Accession Number
NP_001015640.1
Reactivity
Bos taurus, Bovine
Target
Myosin regulatory light polypeptide 9
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MYL12A ELISA Kit (Bovine) (OKEH07563) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MYL12A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MYL12A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MYL12A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Bovine Myosin regulatory light polypeptide 9
NCBI Gene Symbol
MYL12A
Protein Name
Q5E9E2
Gene Name URL
MYL12A
Nucleotide Accession Number
NM_001015640.2
Frequently Asked Questions
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