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LRP8 ELISA Kit (Chicken)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

LDL receptor related protein 8

Gene Aliases

Apolipoprotein E receptor 2; low density lipoprotein receptor-related protein 8, apolipoprotein e receptor; low-density lipoprotein receptor-related protein 8; LR8B; LRP-8; Protein LR8B.

Gene ID

396102

Accession Number

NP_990517.1

Reactivity

Chicken, Gallus gallus

Target

Low-density lipoprotein receptor-related protein 8

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LRP8 ELISA Kit (Chicken) (OKEH07512) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LRP8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LRP8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LRP8 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99% (n = 20)

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Chicken Low-density lipoprotein receptor-related protein 8

NCBI Gene Symbol

LRP8

Protein Name

Q98931

Gene Name URL

LRP8

Nucleotide Accession Number

NM_205186.2

Frequently Asked Questions

More Discoveries

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