What is the catalog number for GNRH1 ELISA Kit (Fish)?

The catalog number for GNRH1 ELISA Kit (Fish) is OKEH07496-96W.

What sizes are available for GNRH1 ELISA Kit (Fish)?

GNRH1 ELISA Kit (Fish) is available in the following size(s): 96 Tests.

Is GNRH1 ELISA Kit (Fish) in stock?

GNRH1 ELISA Kit (Fish) is currently made to order / not held in immediate stock. Contact us for lead time.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

GNRH1 ELISA Kit (Fish)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

Prepro-gonadotropin-releasing hormone

Gene Aliases

Gonadotropin-releasing hormone; mdgnrh; medaka-type gonadotropin-releasing hormone; mfGnRH; progonadoliberin I; progonadoliberin-1.

Gene ID

100049266

Accession Number

NP_001098169.1

Reactivity

Fish, Oryzias latipes

Target

Progonadoliberin-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology GNRH1 ELISA Kit (OKEH07496) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-GNRH1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GNRH1 and incubated. The GNRH1 found in the sample or standards competes with the biotinylated GNRH1 for limited binding sites on the immobilized anti-GNRH1 antibody. Excess unbound biotinylated GNRH1 and sample or standard GNRH1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GNRH1 captured in the well and inversely proportional to the amount of GNRH1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol