What is the catalog number for KITLG ELISA Kit (Dog)?

The catalog number for KITLG ELISA Kit (Dog) is OKEH07312-96W.

What sizes are available for KITLG ELISA Kit (Dog)?

KITLG ELISA Kit (Dog) is available in the following size(s): 96 Tests.

Is KITLG ELISA Kit (Dog) in stock?

KITLG ELISA Kit (Dog) is currently made to order / not held in immediate stock. Contact us for lead time.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

What is the difference between this product and a similar catalogue number?

Differences typically involve clone, conjugate, host species, or validated applications. Compare the respective datasheets, or contact us with both catalogue numbers for a direct comparison.

KITLG ELISA Kit (Dog)

Product Specifications

Reproducibility

Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

CAS Number

7732-18-5

Gene Name

KIT ligand

Gene Aliases

710-712; c-kit ligand; CSF; kit ligand; mast cell growth factor; MGF; SCF; Stem cell factor; stem cell factor, CSF.

Gene ID

403507

Accession Number

NP_001012753.1

Reactivity

Canis lupus, Dog

Target

Kit ligand

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology KITLG ELISA Kit (Dog) (OKEH07312) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KITLG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KITLG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KITLG captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 118% (n = 20)

Sensitivity

39pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Dog Kit ligand

NCBI Gene Symbol

KITLG

Protein Name

Q06220

Gene Name URL

KITLG

Nucleotide Accession Number

NM_001012735.2

Frequently Asked Questions

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