CD40LG ELISA Kit (Dog)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
CD40 ligand
Gene Aliases
CD154; CD40 ligand; CD40 ligand (TNF superfamily, member 5, hyper-IgM syndrome) ; CD40-L; TNFSF5; TNLG8B; tumor necrosis factor (ligand) superfamily, member 5; tumor necrosis factor ligand 8B; Tumor necrosis factor ligand superfamily member 5.
Gene ID
403468
Accession Number
NP_001002981.1
Reactivity
Canis lupus, Dog
Target
CD40 ligand
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD40LG ELISA Kit (Dog) (OKEH07309) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD40LG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD40LG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD40LG captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)
Sensitivity
0.15ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Dog CD40 ligand
NCBI Gene Symbol
CD40LG
Protein Name
O97626
Gene Name URL
CD40LG
Nucleotide Accession Number
NM_001002981.1
Curated Selection
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