CCN2 ELISA Kit (Bovine)
Product Specifications
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Cellular communication network factor 2
Gene Aliases
CCN family member 2; connective tissue growth factor; CTGF.
Gene ID
281103
Accession Number
NP_776455.1
Reactivity
Bos taurus, Bovine
Target
Connective tissue growth factor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CCN2 ELISA Kit (Bovine) (OKEH07219) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCN2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCN2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83% (n = 20)
Sensitivity
0.15ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Bovine CCN family member 2
NCBI Gene Symbol
CCN2
Protein Name
O18739
Gene Name URL
CCN2
Nucleotide Accession Number
NM_174030.2
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC470630-100 1x 100 µL
Mucin 1 / EMA / Episialin / CD227 (VU-2G7), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC472946-100 1x 100 µL
TLE1 (Synovial Sarcoma Marker) (TLE1/2946R), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC880768-500 1x 500 µL
GnRH-Receptor (LHRH Receptor) (GNRHR/768), CF488A conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC941448-100 1x 100 µL
Factor XIIIa (Coagulation Factor XIIIA Chain) (F13A1/1448), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 