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WARS ELISA Kit (Mouse)

Selected Variant

OKEH07208-96W

Size: 96 Well

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Tryptophanyl-tRNA synthetase

Gene Aliases

TrpRS; tryptophan--tRNA ligase, cytoplasmic; Tryptophanyl-tRNA synthetase; tryptophanyl-tRNA synthetase, cytoplasmic; Wars1; WRS.

Gene ID

22375

Accession Number

NP_001157786

Reactivity

Mouse, Mus musculus

Target

Tryptophan--tRNA ligase, cytoplasmic

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Wars ELISA Kit (Mouse) (OKEH07208) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Wars has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Wars is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Wars captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into matrix = 103.4%

Sensitivity

0.088 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Tryptophan--tRNA ligase, cytoplasmic

NCBI Gene Symbol

Wars

Protein Name

P32921

Gene Name URL

Wars

Nucleotide Accession Number

NM_001164314

Price

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