WARS ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Tryptophanyl-tRNA synthetase 1
Gene Aliases
Epididymis secretory sperm binding protein; GAMMA-2; HMN9; hWRS; IFI53; IFP53; interferon-induced protein 53; trpRS; tryptophan tRNA ligase 1, cytoplasmic; tryptophan--tRNA ligase, cytoplasmic; Tryptophanyl-tRNA synthetase; WARS.
Gene ID
7453
Accession Number
NP_004175
Reactivity
Homo sapiens, Human
Target
Tryptophan--tRNA ligase, cytoplasmic
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology WARS ELISA Kit (Human) (OKEH07207) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WARS has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WARS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WARS captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.321-20ng/mL
Recovery
Mean recovery when spiking into matrix = 89.9%
Sensitivity
0.183 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Tryptophan--tRNA ligase, cytoplasmic
NCBI Gene Symbol
WARS1
Protein Name
P23381
Gene Name URL
WARS1
Nucleotide Accession Number
NM_004184
Frequently Asked Questions
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