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STAT4 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Signal transducer and activator of transcription 4

Gene Aliases

Signal transducer and activator of transcription 4; signal transducer and activator of transcription 4 variant 3; SLEB11.

Gene ID

6775

Accession Number

NP_001230764

Reactivity

Homo sapiens, Human

Target

Signal transducer and activator of transcription 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology STAT4 ELISA Kit (Human) (OKEH07174) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for STAT4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for STAT4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample STAT4 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into matrix = 108.3%

Sensitivity

0.168 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Signal transducer and activator of transcription 4

NCBI Gene Symbol

STAT4

Protein Name

Q14765

Gene Name URL

STAT4

Nucleotide Accession Number

NM_001243835

Frequently Asked Questions

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