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SLC6A4 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Solute carrier family 6 member 4

Gene Aliases

5HT transporter;5HTT;5-HTT;5-HTTLPR;5-hydroxytryptamine (serotonin) transporter; hSERT; HTT; Na+/Cl- dependent serotonin transporter; OCD1; serotonin transporter 1; SERT; SERT1; sodium-dependent serotonin transporter; solute carrier family 6 (neurotransmitter transporter), member 4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4; Solute carrier family 6 member 4.

Gene ID

6532

Accession Number

NP_001036

Reactivity

Homo sapiens, Human

Target

Sodium-dependent serotonin transporter

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SLC6A4 ELISA Kit (Human) (OKEH07159) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC6A4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC6A4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC6A4 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into matrix = 105%

Sensitivity

7.9 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Sodium-dependent serotonin transporter

NCBI Gene Symbol

SLC6A4

Protein Name

P31645

Gene Name URL

SLC6A4

Nucleotide Accession Number

NM_001045

Frequently Asked Questions

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