RUNX2 ELISA Kit (Mouse)

Acute myeloid leukemia 3 protein; AKV core binding factor; AM; AML3; Cbf; Cbfa; Cbfa1; Cbfa-1; CBF-alpha-1; core binding factor alpha 1; Core-binding factor subunit alpha-1; LS3; Oncogene AML-3; Os; Osf2; osteoblast-specific transcription factor 2; PEA2-alpha A; PEB; PEBP2 alpha A; Pebp2a1; PEBP2aA; Pebpa2a; polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt-related transcription factor 2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit; transcription factor Cbfa1/Osf2.

Aviva Systems Biology Runx2 ELISA Kit (Mouse) (OKEH07140) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Runx2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Runx2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Runx2 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol