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PRDX6 ELISA Kit (Mouse)

Selected Variant

OKEH07122-96W

Size: 96 Well

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Peroxiredoxin 6

Gene Aliases

1-cys;1-Cys peroxiredoxin;1-Cys Prx;1-cysPrx;9430088D19Rik; a; AA690119; acidic calcium-independent phospholipase A2; aiPLA2; antioxidant protein 2; anti-oxidant protein 2, related sequence 3; Aop2; Brp-; Brp-12; CP-; CP-3; glutathione-dependent peroxiredoxin; GP; GPx; LPC acyltransferase 5; LPCAT-5; Ltw; Ltw-; Ltw4; Ltw-4; Lvtw; Lvtw-4; lyso-PC acyltransferase 5; lysophosphatidylcholine acyltransferase 5; N; non-selenium glutathione peroxidase; NSGPx; ORF06; peroxiredoxin 5, related sequence 3; peroxiredoxin 6, related sequence 3; peroxiredoxin-6; Prdx5.

Gene ID

11758

Accession Number

NP_031479

Reactivity

Mouse, Mus musculus

Target

Peroxiredoxin-6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Prdx6 ELISA Kit (Mouse) (OKEH07122) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prdx6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Prdx6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prdx6 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

62.5-4000pg/mL

Recovery

Mean recovery when spiking into matrix = 86.3%

Sensitivity

31.25 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Peroxiredoxin-6

NCBI Gene Symbol

Prdx6

Protein Name

O08709

Gene Name URL

Prdx6

Nucleotide Accession Number

NM_007453

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