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PNOC ELISA Kit (Mouse)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Prepronociceptin

Gene Aliases

N/O; N/OFQ; N23; N23K/N27K; neuropeptide nociceptin 1; Np; Npnc1; OFQ; OFQ/N; orphanin FQ; p; prepronociceptin; proorphanin.

Gene ID

18155

Accession Number

NP_001192004

Reactivity

Mouse, Mus musculus

Target

Prepronociceptin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Pnoc ELISA Kit (Mouse) (OKEH07115) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pnoc has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pnoc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pnoc captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into matrix = 85.7%

Sensitivity

39.6 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Prepronociceptin

NCBI Gene Symbol

Pnoc

Protein Name

Q64387

Gene Name URL

Pnoc

Nucleotide Accession Number

NM_001205075

Frequently Asked Questions

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