PLA2G2A ELISA Kit (Rat)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Phospholipase A2 group IIA
Gene Aliases
Eted enzyme type IIA phospholipase A2; GIIC sPLA2; group IIA phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2, group IIA (platelets, synovial fluid) ; phospholipase A2, membrane associated; platelet phospholipase A2; sPLA2.
Gene ID
29692
Accession Number
NP_113786
Reactivity
Rat, Rattus norvegicus
Target
Phospholipase A2, membrane associated
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pla2g2a ELISA Kit (Rat) (OKEH07110) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pla2g2a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pla2g2a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pla2g2a captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into matrix = 90%
Sensitivity
0.406 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Phospholipase A2, membrane associated
NCBI Gene Symbol
Pla2g2a
Protein Name
P14423
Gene Name URL
Pla2g2a
Nucleotide Accession Number
NM_031598
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
151133 1 Bag (1 Label (s) x 1 Roll)
J20C-2000-2550 Continuous Tapes for Printers
Sign In for Pricing
View Details 
NBP3-28396-100ug 100 µg
CHO Monoclonal HGF Antibody (TAK-701) - Humanized - (Dry Ice)
Sign In for Pricing
View Details