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OVGP1 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Oviductal glycoprotein 1

Gene Aliases

CHIT5; EGP; estrogen-dependent oviduct protein; MUC9; mucin 9; Mucin-9; OGP; oviduct glycoprotein; Oviductal glycoprotein; oviductal glycoprotein 1, 120kDa; oviductin; oviduct-specific glycoprotein.

Gene ID

5016

Accession Number

NP_002548

Reactivity

Homo sapiens, Human

Target

Oviduct-specific glycoprotein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology OVGP1 ELISA Kit (Human) (OKEH07097) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OVGP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OVGP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OVGP1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into matrix = 88.9%

Sensitivity

0.17 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Oviduct-specific glycoprotein

NCBI Gene Symbol

OVGP1

Protein Name

Q12889

Gene Name URL

OVGP1

Nucleotide Accession Number

NM_002557

Frequently Asked Questions

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