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NTRK1 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Neurotrophic receptor tyrosine kinase 1

Gene Aliases

Gp140trk; high affinity nerve growth factor receptor; MTC; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; Oncogene TRK; p140-TrkA; TRK; TRK1; TRK1-transforming tyrosine kinase protein; Trk-A; TRKA; tropomyosin-related kinase A; Tyrosine kinase receptor; tyrosine kinase receptor A.

Gene ID

4914

Accession Number

NP_001007793

Reactivity

Homo sapiens, Human

Target

High affinity nerve growth factor receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NTRK1 ELISA Kit (Human) (OKEH07093) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NTRK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NTRK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NTRK1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into matrix = 96%

Sensitivity

24 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human High affinity nerve growth factor receptor

NCBI Gene Symbol

NTRK1

Protein Name

P04629

Gene Name URL

NTRK1

Nucleotide Accession Number

NM_001007792

Frequently Asked Questions

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