What is the catalog number for MYH4 ELISA Kit (Human)?

The catalog number for MYH4 ELISA Kit (Human) is OKEH07077-96W.

What sizes are available for MYH4 ELISA Kit (Human)?

MYH4 ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is MYH4 ELISA Kit (Human) in stock?

MYH4 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

MYH4 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Myosin heavy chain 4

Gene Aliases

MYH2B; MyHC-2B; MyHC-IIb; myosin heavy chain 2b; Myosin heavy chain 4; myosin heavy chain IIb; myosin heavy chain, skeletal muscle, fetal; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 4, skeletal muscle; myosin-4.

Gene ID

4622

Accession Number

NP_060003

Reactivity

Homo sapiens, Human

Target

Myosin-4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MYH4 ELISA Kit (Human) (OKEH07077) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-MYH4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MYH4 and incubated. The MYH4 found in the sample or standards competes with the biotinylated MYH4 for limited binding sites on the immobilized anti-MYH4 antibody. Excess unbound biotinylated MYH4 and sample or standard MYH4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MYH4 captured in the well and inversely proportional to the amount of MYH4 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol