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MSMB ELISA Kit (Mouse)

Product Specifications

Reproducibility

Inter-Assay CV

Gene Name

Beta-microseminoprotein

Gene Aliases

Beta-; beta-inhibin; beta-microseminoprotein; beta-MSP; PIP; prostate secreted seminal plasma protein; prostate secretory protein of 94 amino acids; prostatic inhibin protein; PS; PSP94; PSP-94.

Gene ID

17695

Accession Number

NP_065622

Reactivity

Mouse, Mus musculus

Target

Beta-microseminoprotein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Msmb ELISA Kit (Mouse) (OKEH07071) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Msmb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Msmb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Msmb captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into matrix = 89.6%

Sensitivity

0.16 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Beta-microseminoprotein

NCBI Gene Symbol

Msmb

Protein Name

O08540

Gene Name URL

Msmb

CAS Number

7732-18-5

Nucleotide Accession Number

NM_020597

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