What is the catalog number for MGMT ELISA Kit (Rat)?

The catalog number for MGMT ELISA Kit (Rat) is OKEH07066-96W.

What sizes are available for MGMT ELISA Kit (Rat)?

MGMT ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is MGMT ELISA Kit (Rat) in stock?

MGMT ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

Is this product species cross-reactive?

Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.

MGMT ELISA Kit (Rat)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

O-6-methylguanine-DNA methyltransferase

Gene Aliases

0-6-methylguanine-DNA methyltransferase;6-O-methylguanine-DNA methyltransferase; methylated-DNA--protein-cysteine methyltransferase; O (6) -alkylguanine-DNA alkyltransferase; O6-alkylguanine-DNA alkyltransferase; O6-methylguanine-DNA methyltranferase; O-6-methylguanine-DNA-alkyltransferase.

Gene ID

25332

Accession Number

NP_036993

Reactivity

Rat, Rattus norvegicus

Target

Methylated-DNA--protein-cysteine methyltransferase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Mgmt ELISA Kit (Rat) (OKEH07066) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mgmt has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mgmt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mgmt captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol