MAT2A ELISA Kit (Rat)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Methionine adenosyltransferase 2A
Gene Aliases
AdoMet synthase 2; adoMet synthetase 2; MAT 2; MAT-II; methionine adenosyltransferase 2; Methionine adenosyltransferase II; methionine adenosyltransferase II, alpha; non-hepatic-type S-adenosylmethionine synthetase; S-adenosylmethionine synthase isoform type-2; Sams2.
Gene ID
171347
Accession Number
NP_599178
Reactivity
Rat, Rattus norvegicus
Target
S-adenosylmethionine synthase isoform type-2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Mat2a ELISA Kit (Rat) (OKEH07058) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mat2a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mat2a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mat2a captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into matrix = 80%
Sensitivity
0.087 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat S-adenosylmethionine synthase isoform type-2
NCBI Gene Symbol
Mat2a
Protein Name
P18298
Gene Name URL
Mat2a
Nucleotide Accession Number
NM_134351
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