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LIPE ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Lipase E, hormone sensitive type

Gene Aliases

AOMS4; FPLD6; hormone-sensitive lipase; HSL; LHS; lipase, hormone-sensitive; monoacylglycerol lipase LIPE; REH; retinyl ester hydrolase.

Gene ID

3991

Accession Number

NP_005348

Reactivity

Homo sapiens, Human

Target

Hormone-sensitive lipase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LIPE ELISA Kit (Human) (OKEH07041) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LIPE has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LIPE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LIPE captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

1.56-100U/L

Recovery

Mean recovery when spiking into matrix = 94.9%

Sensitivity

0.89U/L

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Hormone-sensitive lipase

NCBI Gene Symbol

LIPE

Protein Name

Q05469

Gene Name URL

LIPE

Nucleotide Accession Number

NM_005357

Frequently Asked Questions

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