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LDHC ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Lactate dehydrogenase C

Gene Aliases

Cancer/testis antigen 32; CT32; lactate dehydrogenase c variant 1; lactate dehydrogenase c variant 3; lactate dehydrogenase c variant 4; lactate dehydrogenase c variant 5; lactate dehydrogenase C4; LDH testis subunit; LDH3; LDH-C; LDHX; LDH-X; L-lactate dehydrogenase C chain; testis secretory sperm-binding protein Li 234P.

Gene ID

3948

Accession Number

NP_002292

Reactivity

Homo sapiens, Human

Target

L-lactate dehydrogenase C chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LDHC ELISA Kit (Human) (OKEH07036) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LDHC has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LDHC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LDHC captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

1.56-100U/L

Recovery

Mean recovery when spiking into matrix = 106%

Sensitivity

0.39U/L

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human L-lactate dehydrogenase C chain

NCBI Gene Symbol

LDHC

Protein Name

P07864

Gene Name URL

LDHC

Nucleotide Accession Number

NM_002301

Frequently Asked Questions

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