What is the catalog number for LCAT ELISA Kit (Mouse)?

The catalog number for LCAT ELISA Kit (Mouse) is OKEH07035-96W.

What sizes are available for LCAT ELISA Kit (Mouse)?

LCAT ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is LCAT ELISA Kit (Mouse) in stock?

LCAT ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

LCAT ELISA Kit (Mouse)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Lecithin cholesterol acyltransferase

Gene Aliases

1-alkyl-2-acetylglycerophosphocholine esterase; AI046659; D8Wsu61; D8Wsu61e; Lecithin-cholesterol acyltransferase; lecithin-cholesterol acyltransferase Lcat; PAF acetylhydrolase; phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase; platelet-activating factor acetylhydrolase.

Gene ID

16816

Accession Number

NP_032516

Reactivity

Mouse, Mus musculus

Target

Phosphatidylcholine-sterol acyltransferase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Lcat ELISA Kit (Mouse) (OKEH07035) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lcat has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lcat is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lcat captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol