HSP90AA1 ELISA Kit (Rat)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Heat shock protein 90 alpha family class A member 1, heat shock protein HSP 90-alpha
Gene Aliases
Heat shock 86 kDa; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90, alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; heat shock protein HSP 90-alpha; HSP 86; Hsp86; Hsp90; Hspca.
Gene ID
103692716, 299331
Accession Number
NP_786937
Reactivity
Rat, Rattus norvegicus
Target
Heat shock protein HSP 90-alpha
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hsp90aa1 ELISA Kit (Rat) (OKEH06999) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hsp90aa1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hsp90aa1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hsp90aa1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into matrix = 109%
Sensitivity
0.079 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Heat shock protein HSP 90-alpha
NCBI Gene Symbol
Hsp90aa1, LOC103692716
Protein Name
P82995
Gene Name URL
Hsp90aa1, LOC103692716
Nucleotide Accession Number
NM_175761
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