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GCG ELISA Kit (Pig)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Glucagon

Gene Aliases

GLP-1; glucagon; proglucagon; pro-glucagon.

Gene ID

397595

Accession Number

NP_999489

Reactivity

Porcine, Sus scrofa

Target

Glucagon

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Gcg ELISA Kit (Pig) (OKEH06981) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gcg has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gcg is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gcg captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

31.2-2000pg/mL

Sensitivity

7.5 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Pig Glucagon

NCBI Gene Symbol

GCG

Protein Name

P01274

Gene Name URL

GCG

Nucleotide Accession Number

NM_214324

Frequently Asked Questions

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