FMR1NB ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
FMR1 neighbor
Gene Aliases
Cancer/testis antigen 37; CT37; fragile X mental retardation 1 neighbor protein; NYSAR35; NY-SAR-35; sarcoma antigen NY-SAR-35.
Gene ID
158521
Accession Number
NP_689791
Reactivity
Homo sapiens, Human
Target
Fragile X mental retardation 1 neighbor protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FMR1NB ELISA Kit (Human) (OKEH06975) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FMR1NB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FMR1NB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FMR1NB captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into matrix = 106%
Sensitivity
0.398 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Fragile X mental retardation 1 neighbor protein
NCBI Gene Symbol
FMR1NB
Protein Name
Q8N0W7
Gene Name URL
FMR1NB
Nucleotide Accession Number
NM_152578
Frequently Asked Questions
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