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DRD2 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Dopamine receptor D2

Gene Aliases

D (2) dopamine receptor; D2DR; D2R; dopamine D2 receptor; seven transmembrane helix receptor.

Gene ID

1813

Accession Number

NP_000786

Reactivity

Homo sapiens, Human

Target

D (2) dopamine receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DRD2 ELISA Kit (Human) (OKEH06951) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DRD2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DRD2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DRD2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into matrix = 87.9%

Sensitivity

0.156 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human D (2) dopamine receptor

NCBI Gene Symbol

DRD2

Protein Name

P14416

Gene Name URL

DRD2

Nucleotide Accession Number

NM_000795

Frequently Asked Questions

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