DPYD ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Dihydropyrimidine dehydrogenase
Gene Aliases
DHP; DHPDHASE; dihydropyrimidine dehydrogenase [NADP (+) ]; dihydrothymine dehydrogenase; dihydrouracil dehydrogenase; DPD.
Gene ID
1806
Accession Number
NP_000101
Reactivity
Homo sapiens, Human
Target
Dihydropyrimidine dehydrogenase [NADP (+) ]
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DPYD ELISA Kit (Human) (OKEH06948) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DPYD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DPYD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DPYD captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into matrix = 85%
Sensitivity
0.089 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Dihydropyrimidine dehydrogenase [NADP (+) ]
NCBI Gene Symbol
DPYD
Protein Name
Q12882
Gene Name URL
DPYD
Nucleotide Accession Number
NM_000110
Frequently Asked Questions
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