What is the catalog number for CYP1B1 ELISA Kit (Human)?

The catalog number for CYP1B1 ELISA Kit (Human) is OKEH06933-96W.

What sizes are available for CYP1B1 ELISA Kit (Human)?

CYP1B1 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is CYP1B1 ELISA Kit (Human) in stock?

CYP1B1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What should I check when this product first arrives?

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CYP1B1 ELISA Kit (Human)

Selected Variant

OKEH06933-96W

Size: 96 Well

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Cytochrome P450 family 1 subfamily B member 1

Gene Aliases

Aryl hydrocarbon hydroxylase; ASGD6; CP1B; CYPIB1; cytochrome P450 1B1; cytochrome P450, family 1, subfamily B, polypeptide 1; cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile) ; dioxin-inducible cytochrome p450; flavoprotein-linked monooxygenase; GLC3A; hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; P4501B1; xenobiotic monooxygenase.

Gene ID

1545

Accession Number

NP_000095

Reactivity

Homo sapiens, Human

Target

Cytochrome P450 1B1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology CYP1B1 ELISA Kit (Human) (OKEH06933) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-CYP1B1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CYP1B1 and incubated. The CYP1B1 found in the sample or standards competes with the biotinylated CYP1B1 for limited binding sites on the immobilized anti-CYP1B1 antibody. Excess unbound biotinylated CYP1B1 and sample or standard CYP1B1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CYP1B1 captured in the well and inversely proportional to the amount of CYP1B1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into matrix = 109%

Sensitivity

0.407 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Cytochrome P450 1B1

NCBI Gene Symbol

CYP1B1

Protein Name

Q16678

Gene Name URL

CYP1B1

Nucleotide Accession Number

NM_000104

Price

Frequently Asked Questions

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