What is the catalog number for CYP11A1 ELISA Kit (Rat)?

The catalog number for CYP11A1 ELISA Kit (Rat) is OKEH06930-96W.

What sizes are available for CYP11A1 ELISA Kit (Rat)?

CYP11A1 ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is CYP11A1 ELISA Kit (Rat) in stock?

CYP11A1 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

CYP11A1 ELISA Kit (Rat)

Selected Variant

OKEH06930-96W

Size: 96 Well

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Cytochrome P450, family 11, subfamily a, polypeptide 1

Gene Aliases

Cholesterol desmolase; cholesterol side-chain cleavage cytochrome P450; cholesterol side-chain cleavage enzyme, mitochondrial; Cyp11a; Cypxia1; cytochrome P450 11a cholesterol side chain cleavage; cytochrome P450 11A1; cytochrome P450 family 11 subfamily a; cytochrome P450 side-chain cleavage enzyme; cytochrome P450 subfamily 11A; cytochrome P450 (scc) ; P450 (scc) ; P450scc.

Gene ID

29680

Accession Number

NP_058982

Reactivity

Rat, Rattus norvegicus

Target

Cholesterol side-chain cleavage enzyme, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Cyp11a1 ELISA Kit (Rat) (OKEH06930) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp11a1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cyp11a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp11a1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into matrix = 99%

Sensitivity

0.406 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Cholesterol side-chain cleavage enzyme, mitochondrial

NCBI Gene Symbol

Cyp11a1

Protein Name

P14137

Gene Name URL

Cyp11a1

Nucleotide Accession Number

NM_017286

Price

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