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CUBN ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Cubilin

Gene Aliases

460 kDa receptor; cubilin; cubilin (intrinsic factor-cobalamin receptor) ; cubilin precursor variant 1; cubilin precursor variant 2; cubilin precursor variant 3; gp280; IFCR; IGS; IGS1; intestinal intrinsic factor receptor; Intrinsic factor-cobalamin receptor; intrinsic factor-vitamin B12 receptor; MGA1.

Gene ID

8029

Accession Number

NP_001072

Reactivity

Homo sapiens, Human

Target

Cubilin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CUBN ELISA Kit (Human) (OKEH06922) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CUBN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CUBN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CUBN captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into matrix = 92.9%

Sensitivity

0.081 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Cubilin

NCBI Gene Symbol

CUBN

Protein Name

O60494

Gene Name URL

CUBN

Nucleotide Accession Number

NM_001081

Frequently Asked Questions

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