CUBN ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Cubilin
Gene Aliases
460 kDa receptor; cubilin; cubilin (intrinsic factor-cobalamin receptor) ; cubilin precursor variant 1; cubilin precursor variant 2; cubilin precursor variant 3; gp280; IFCR; IGS; IGS1; intestinal intrinsic factor receptor; Intrinsic factor-cobalamin receptor; intrinsic factor-vitamin B12 receptor; MGA1.
Gene ID
8029
Accession Number
NP_001072
Reactivity
Homo sapiens, Human
Target
Cubilin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CUBN ELISA Kit (Human) (OKEH06922) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CUBN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CUBN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CUBN captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into matrix = 92.9%
Sensitivity
0.081 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Cubilin
NCBI Gene Symbol
CUBN
Protein Name
O60494
Gene Name URL
CUBN
Nucleotide Accession Number
NM_001081
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog