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ANXA1 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Annexin A1

Gene Aliases

Annexin A1; annexin I; annexin-1; Anx; Anx-1; Anx-A1; C430014K04Rik; calpactin II; calpactin-2; chromobindin-9; lipocortin 1; lipocortin I; Lp; Lpc; Lpc1; Lpc-1; p35; phospholipase A2 inhibitory protein.

Gene ID

16952

Accession Number

NP_034860

Reactivity

Mouse, Mus musculus

Target

Annexin A1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Anxa1 ELISA Kit (Mouse) (OKEH06853) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Anxa1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Anxa1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Anxa1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into matrix = 114%

Sensitivity

0.16 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Annexin A1

NCBI Gene Symbol

Anxa1

Protein Name

P10107

Gene Name URL

Anxa1

Nucleotide Accession Number

NM_010730

Frequently Asked Questions

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