What is the catalog number for ANPEP ELISA Kit (Human)?

The catalog number for ANPEP ELISA Kit (Human) is OKEH06852-96W.

What sizes are available for ANPEP ELISA Kit (Human)?

ANPEP ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is ANPEP ELISA Kit (Human) in stock?

ANPEP ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

ANPEP ELISA Kit (Human)

Selected Variant

OKEH06852-96W

Size: 96 Tests

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Alanyl aminopeptidase, membrane

Gene Aliases

Alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; aminopeptidase M; aminopeptidase N; AP-M; APN; AP-N; CD13; GP150; hAPN; LAP1; membrane alanyl aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13; P150; PEPN.

Gene ID

290

Accession Number

NP_001141

Reactivity

Homo sapiens, Human

Target

Aminopeptidase N

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ANPEP ELISA Kit (Human) (OKEH06852) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANPEP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANPEP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANPEP captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into matrix = 85%

Sensitivity

0.085 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Aminopeptidase N

NCBI Gene Symbol

ANPEP

Protein Name

P15144

Gene Name URL

ANPEP

Nucleotide Accession Number

NM_001150

Price

Frequently Asked Questions

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