What is the catalog number for AMY2 ELISA Kit (Rat)?

The catalog number for AMY2 ELISA Kit (Rat) is OKEH06850-96W.

What sizes are available for AMY2 ELISA Kit (Rat)?

AMY2 ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is AMY2 ELISA Kit (Rat) in stock?

AMY2 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

Do you offer bulk or custom quantities of this product?

Yes. Ordering two or more units, or combining this with other products, usually qualifies for a discount depending on quantity. Contact our sales team with your required volume for a custom quote.

AMY2 ELISA Kit (Rat)

Selected Variant

OKEH06850-96W

Size: 96 Well

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Amylase 2a3

Gene Aliases

1,4-alpha-D-glucan glucanohydrolase; Amy2; amylase 2, pancreatic; PA; pancreatic alpha-amylase; pancreatic alpha-amylase-like; pancreatic amylase.

Gene ID

497039

Accession Number

NP_113690

Reactivity

Rat, Rattus norvegicus

Target

Pancreatic alpha-amylase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology Amy2 ELISA Kit (Rat) (OKEH06850) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Amy2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Amy2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Amy2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into matrix = 89%

Sensitivity

0.092 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Pancreatic alpha-amylase

NCBI Gene Symbol

Amy2a3

Protein Name

P00689

Gene Name URL

Amy2a3

Nucleotide Accession Number

NM_031502

Price

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) (CDH1/3256), CF488A conjugate, 0.1mg/mL

BNC883256-100 1x 100 µL