ACTN1 ELISA Kit (Rat)
Product Specifications
Reproducibility
Inter-Assay CV
Gene Name
Actinin, alpha 1
Gene Aliases
Alpha-actinin cytoskeletal isoform; alpha-actinin-1; F-actin cross-linking protein; non-muscle alpha-actinin-1.
Gene ID
81634
Accession Number
NP_112267
Reactivity
Rat, Rattus norvegicus
Target
Alpha-actinin-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Actn1 ELISA Kit (Rat) (OKEH06841) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Actn1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Actn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Actn1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into matrix = 94%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Alpha-actinin-1
NCBI Gene Symbol
Actn1
Protein Name
Q9Z1P2
Gene Name URL
Actn1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_031005
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