MMP23B ELISA Kit (Human)
Product Specifications
Reproducibility
Intra-Assay Reproducibility CV% < 7.5% (n=20) < br>Inter-Assay Reproducibility CV% < 9.7% (n=24)
CAS Number
7732-18-5
Gene Name
Matrix metallopeptidase 23B
Gene Aliases
Femalysin; matrix metalloproteinase 22; matrix metalloproteinase 23B; matrix metalloproteinase in the female reproductive tract; matrix metalloproteinase-21; Matrix metalloproteinase-22; matrix metalloproteinase-23; MIFR; MIFR-1; MMP-21; MMP22; MMP-22; MMP-23; MMP23A.
Gene ID
8510
Accession Number
NP_008914
Reactivity
Homo sapiens, Human
Target
Matrix metalloproteinase-23
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MMP23B ELISA Kit (Human) (OKEH06830) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP23B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP23B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP23B captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into serum = 100%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Matrix metalloproteinase-23
NCBI Gene Symbol
MMP23B
Protein Name
O75900
Gene Name URL
MMP23B
Nucleotide Accession Number
NM_006983
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
