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ADAM10 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Intra-Assay Reproducibility CV% < 7.1% (n=20) < br>Inter-Assay Reproducibility CV% < 9.2% (n=24)

CAS Number

7732-18-5

Gene Name

A disintegrin and metallopeptidase domain 10

Gene Aliases

1700031C13Rik; a disintegrin and metalloprotease domain (ADAM) 10; disintegrin and metalloproteinase domain-containing protein 10; kuz; kuzb; kuzbanian; kuzbanian protein homolog; MADM; mammalian disintegrin-metalloprotease.

Gene ID

11487

Accession Number

NP_031425

Reactivity

Mouse, Mus musculus

Target

Disintegrin and metalloproteinase domain-containing protein 10

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Adam10 ELISA Kit (Mouse) (OKEH06827) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adam10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Adam10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adam10 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.081 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Disintegrin and metalloproteinase domain-containing protein 10

NCBI Gene Symbol

Adam10

Protein Name

O35598

Gene Name URL

Adam10

Nucleotide Accession Number

NM_007399

Frequently Asked Questions

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