MYOF ELISA Kit (Mouse)
Product Specifications
Reproducibility
Intra-Assay Reproducibility CV% < 6.9% (n=20) < br>Inter-Assay Reproducibility CV% < 9.3% (n=24)
CAS Number
7732-18-5
Gene Name
Myoferlin
Gene Aliases
2310004N10Rik;2310051D19Rik; E030042N20Rik; Fer1; Fer1l3; fer-1-like protein 3; myoferlin.
Gene ID
226101
Accession Number
NP_001093104
Reactivity
Mouse, Mus musculus
Target
Myoferlin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Myof ELISA Kit (Mouse) (OKEH06777) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Myof has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Myof is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Myof captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/ml
Recovery
Mean recovery when spiking into serum = 99.1%
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
Myof
Protein Name
Q69ZN7
Gene Name URL
Myof
Nucleotide Accession Number
NM_001099634
Frequently Asked Questions
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