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MYOF ELISA Kit (Mouse)

Product Specifications

Reproducibility

Intra-Assay Reproducibility CV% < 6.9% (n=20) < br>Inter-Assay Reproducibility CV% < 9.3% (n=24)

CAS Number

7732-18-5

Gene Name

Myoferlin

Gene Aliases

2310004N10Rik;2310051D19Rik; E030042N20Rik; Fer1; Fer1l3; fer-1-like protein 3; myoferlin.

Gene ID

226101

Accession Number

NP_001093104

Reactivity

Mouse, Mus musculus

Target

Myoferlin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Myof ELISA Kit (Mouse) (OKEH06777) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Myof has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Myof is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Myof captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78-50ng/ml

Recovery

Mean recovery when spiking into serum = 99.1%

Sensitivity

0.78 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

Myof

Protein Name

Q69ZN7

Gene Name URL

Myof

Nucleotide Accession Number

NM_001099634

Frequently Asked Questions

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