B4GALT5 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Intra-Assay Reproducibility CV% < 5.4% (n=20) < br>Inter-Assay Reproducibility CV% < 7.0% (n=24)
CAS Number
7732-18-5
Gene Name
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5
Gene Aliases
9430078I07Rik; AW049941; AW539721; b4Gal-T5; beta-1,4-galactosyltransferase 5; beta-1,4-galactosyltransferase V; beta-1,4-GalT II; beta-1,4-GalTase 5; beta4Gal-T5; glucosylceramide beta-1,4-galactosyltransferase; lacCer synthase; lactosylceramide synthase; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5.
Gene ID
56336
Accession Number
NP_062809
Reactivity
Mouse, Mus musculus
Target
Beta-1,4-galactosyltransferase 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology B4galt5 ELISA Kit (Mouse) (OKEH06776) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for B4galt5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for B4galt5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample B4galt5 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/ml
Recovery
Mean recovery when spiking into serum = 101.5%
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
B4galt5
Protein Name
Q9JMK0
Gene Name URL
B4galt5
Nucleotide Accession Number
NM_019835
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