B4GALT5 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Intra-Assay Reproducibility CV% < 5.4% (n=20) < br>Inter-Assay Reproducibility CV% < 7.0% (n=24)

CAS Number

7732-18-5

Gene Name

UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5

Gene Aliases

9430078I07Rik; AW049941; AW539721; b4Gal-T5; beta-1,4-galactosyltransferase 5; beta-1,4-galactosyltransferase V; beta-1,4-GalT II; beta-1,4-GalTase 5; beta4Gal-T5; glucosylceramide beta-1,4-galactosyltransferase; lacCer synthase; lactosylceramide synthase; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5.

Gene ID

56336

Accession Number

NP_062809

Reactivity

Mouse, Mus musculus

Target

Beta-1,4-galactosyltransferase 5

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology B4galt5 ELISA Kit (Mouse) (OKEH06776) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for B4galt5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for B4galt5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample B4galt5 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol