What is the catalog number for HOGA1 ELISA Kit (Bovine)?

The catalog number for HOGA1 ELISA Kit (Bovine) is OKEH06684-96W.

What sizes are available for HOGA1 ELISA Kit (Bovine)?

HOGA1 ELISA Kit (Bovine) is available in the following size(s): 96 Well.

Is HOGA1 ELISA Kit (Bovine) in stock?

HOGA1 ELISA Kit (Bovine) is currently made to order / not held in immediate stock. Contact us for lead time.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

HOGA1 ELISA Kit (Bovine)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

4-hydroxy-2-oxoglutarate aldolase 1

Gene Aliases

4-hydroxy-2-oxoglutarate aldolase, mitochondrial; C10orf65; DHDPSL; DHDPS-like protein; Dihydrodipicolinate synthase-like; dihydrodipicolinate synthase-like, mitochondrial; N-acetylneuraminate pyruvate lyase 2 (putative) ; NPL2; probable 2-keto-4-hydroxyglutarate aldolase; probable 4-hydroxy-2-oxoglutarate aldolase, mitochondrial; probable KHG-aldolase.

Gene ID

506001

Accession Number

NP_001068705

Reactivity

Bos taurus, Bovine

Target

4-hydroxy-2-oxoglutarate aldolase, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Hoga1 ELISA Kit (Bovine) (OKEH06684) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hoga1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hoga1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hoga1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.78-50ng/ml

Sensitivity

0.28 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Bovine 4-hydroxy-2-oxoglutarate aldolase, mitochondrial

NCBI Gene Symbol

HOGA1

Protein Name

Q0P5I5

Gene Name URL

HOGA1

Nucleotide Accession Number

NM_001075237

Frequently Asked Questions

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